Showing 4 results for Rezaian
M Rezaian, F Bagheri , Sh Farnia , Z Babai ,
Volume 1, Issue 3 (7 2003)
Abstract
In a survey from 1999 to 2001, a total of 354 samples of soil and water were colleted from different areas and examined for the presence of AcanthamoebaandNaegleria spp. After sieving, filtration and centrifugation, samples were examined for the free living protozoa ( amphizoic amoeba ). Concurrently, a sediment of each sample was cultured in the non-nutrient agar medium enriched by E.coli. In the end, 10 Acanthomoeba spp. and 3 Naegleria sp. were isolated. Besides, we diagnosed one case of human Acanthomoeba infection in a person residing in the area.
Gh Asgari , M Nateghpour , M Rezaian ,
Volume 1, Issue 3 (7 2003)
Abstract
To determine the prevalence of various intestinal parasitic infections, we examined 966randomly collected stool specimens from urban areas and 569 such samples from the ruralregions. These were examined using formalin-ether sedimentation and direct smearmethods. From the total of 1535 specimens, 143 that belonged to 1-6 years old childrenwere examined by scatch tape method.The results indicated that 53.2% of the subjects were infected with intestinal protozoa andhelminths with the following prevalence rates:Entamoeba histolytica 9.6%, E. coli 16%, E.hartmanni 7%, Endolimax nana 2.6%,Iodomoeba biitschlii 1.8%, Dientamoeba fragilis 1.5%, Chi/omastix mesnili 0.4%), Giardialamblia 18.8%, Blastocysts hominis 16.5%, Dicrocoelium dendriticum 0.1%, Taeniasaginata 0.2%, Hymenolopis nana 1.4%, Ascaris lambricodies 0.3%, Enterobiusvermincularis (using scatch tape method) 0.7%, E.vermicularis (using formalin - ethermethod) 28.7%,Trichostrongylusspp.0.1%,Strongyhidessiercorials 0.3% and Trichuristrichiura 0.1%.Rural people were significantly more likely to bear helminthic infections than urbanresidents (4.9% versus 2.1%).E.histofytica wasmore prevalent among men (11% versus 7.1%) and, interestingly,age-specific infection rates for giardiasis and amebiasis showed contrasting patterns in thisstudy
M Rezaian , H Hooshyar ,
Volume 4, Issue 4 (4 2006)
Abstract
Backgroun and Aim: Differential diagnosis of Entamoeba histolytica and Entamoeba dispar is important in clinical and epidemiological studies. The two organisms are morphologically identical but they differ in their genetics, biochemistry, and pathogenicity. The present study was carried out with the aim of distinguishing the two species and determining the prevalence of each organism in the rural areas of Ahwaz and Hamidieh.
Material and Methods: A total of 782 stool specimens were randomly collected and examined by formalin-ether concentration and direct methods. Twenty-one isolates of E. histolytica/ E. dispar were successfully cultured on Robinson's medium. DNA was extracted by the phenol/chloroform method and identified by PCR-RFLP after digestion with HinfI.
Results: Over 75% of the individuals were infected with at least one of the intestinal parasites. Entamoeba coli infection rates were very high (51.9%) among the population, while only 0.76% of individuals were positive for Dientamoeba fragilis. Sixty-five individual (8/3%) were infected with E. histolytica /E. dispar.
The PCR-RFLP showed that 19 samples (90.48%) were positive for E. dispar one sample (4.76%) was positive for E. histolytica and another sample (4.76%) showed mixed infection.
Conclusion: These findings show that the nonpathogenic E. dispar is predominant in Ahwaz and Hamidieh rural area.
S Shojaee , H Keshavarz , M Rezaian , M Mohebali , N Mohajeri , Z Garossi ,
Volume 5, Issue 1 (2 2007)
Abstract
Background and Aim: Toxoplasma gondii is a protozoan parasite infecting humans and warm-blooded animals. Toxoplasmosis in pregnancy could cause neurologic disorders in the fetus. In immunocompromised hosts, the infection can be reactivated with life-threatening consequences. Detection of the parasite or its components would constitute a better definition of acute infection.
Material and Methods: To detect T. gondii antigen and DNA, twenty serum samples in the acute phase of infection were tested. Polyclonal antibodies were isolated from immunized rabbits and SDS-page and immunoblotting were performed. Also, PCR was done with amplification of the B1 gene with two primers.
Results: In one patient T. gondii antigen band with a molecular weight of 30 kDa was detected. Parasitemia was detected in the same patient and the 570 bp amplified DNA fragment was isolated. Others had negative results in both immunoblotting and PCR. The patient with positive results had been infected accidentally with a rather virulent C56 strain in the laboratory.
Conclusion: The results indicate that antigen and DNA of T. gondii can be detected during the short acute phase of infection.
