Vida Saghari, Hossein Jalali, Nabi Shariatifar, Mona Belandi,
Volume 23, Issue 2 (9-2025)
Abstract
Background and Aim: Edible films represent an innovative solution for meat preservation, helping to reduce spoilage and extend shelf life. This study aimed to investigate the effect of edible films containing whey protein and free-form or Pickering emulsion of Trachyspermum copticum L. essential oil on the properties of beef during refrigerated storage.
Materials and Methods: Edible films containing whey protein and T. copticum L. essential oil (in free and Pickering emulsion forms) were prepared. Their physical, microbial, and chemical properties, as well as sensory attributes, were evaluated.
Results: Physical tests demonstrated successful film preparation, with the average zeta diameter of nanoliposomes ranging from 73.91 to 110.53 nm, polydispersity index (PDI) from 0.451 to 0.471, zeta potential from −5.12 mV to −3.64 mV, and encapsulation efficiency from 49.61% to 64.23%. The best performance in microbial, chemical, and sensory evaluations was observed in the treatment containing whey protein with nano-essential oil (Pickering emulsion), compared to the control and free essential oil samples.
Conclusion: The results indicate that edible films containing whey protein and nano-essential oil of T. copticum L. can be an effective strategy for storing beef under refrigerated conditions, improving product shelf life.
