A Mardani , H Keshavarz ,
Volume 2, Issue 3 (6-2004)
Abstract
Toxoplasmosis is a zoonosis of broad geographic distribution. This disease is caused by infection with the protozoan parasite Toxoplasma gondii. The most important ways to diagnose the disease are the serological techniques such as IFA and ELISA. The aim of this consideration, was comparison between the two methods, IFA and ELISA, in order to determine the incidence of antibody against Toxoplasma in pregnant women. In this descriptive-cross sectional study, from 600 pregnant woman whom have referred to Alzzahra and Izadi maternity hospitals of Qom, during four months (from Sep.2001 to Jan.2002), the specimen of blood have been prepared. After bloodletting and parting of serum, IFA and ELISA have tested its specimens. From 600 specimens of serum, from the tested mothers by IgG-ELISA method, 257 individuals (%42.8) and by IgG-IFA method, 246 individuals (%41) had Specific IgG antibody. Also, The comparison between the two methods, IFA and ELISA, demonstrates that 246 cases (%41) were positive in both methods, and 343 cases (%57.2) were negative in both methods, and only 11 cases (%1.8) were positive in ELISA and negative in IFA technique. In this study, the effect of different factors has been studied, in which deal with the prevalence rate of Toxoplasma infection. Although, these results shows that transmission of Toxoplasma infection in this city, like other parts of world and Iran has been done, but considerable percent of pregnant women (%57.2 by ELISA method and %59 by IFA method) lacking any type of acquired immunity against of this infection. Therefore, considering secure status and accomplishing exclusive experiments before marriage and also training and informing the people of region especially pregnant women with educational programs and supervision in pregnant period is necessary. On the other hand, with regard to the rate of concordance of the two tests (%98.2), The ELISA because of its high sensitivity and specificity, easier technique and lower expense it is preferred in order to screening Toxoplasma infection.
Arezu Amirali, Ameneh Elikaei, Roxana Mansour Ghanaie, Idesbald Boone, Abdollah Karimi, Tim Eckmanns, Andreas Lutz Jansen, Fatemeh Fallah, Noushin Marhamati, Niloofar Pashaei, Shahriar Janbazi, Ahmad Reza Shamshiri, Hamid Reza Baradaran, Mohammad Hossein Rostami, Masoud Alebouyeh,
Volume 19, Issue 4 (3-2022)
Abstract
Background and Aim: The objective of this study was to determine the seroepidemiological history of SARS-CoV-2 infection among asymptomatic children in Tehran.
Materials and Methods: Blood samples of children younger than 14 years old were collected during the period autumn-winter 2020 and spring 2021 and tested for SARS-CoV-2 IgG antibody using the EUROIMMUN ELISA kit. In addition, questionnaires were used to collect demographic and infection status information in the participants. Data were analyzed using the SPSS software.
Results: Out of the 1142 children collected from the children with no COVID-19 symptoms, 33.3% (381/1142) were found to have had a history of SARS-CoV-2. The positive samples in girls and boys were 34.1% and 33.03%, respectively. Analysis of the data showed no statistically significant differences between the infection rate on the one hand and age, family size, underlying diseases, gender or occupations of the family members on the other hand. In addition, the infection rate was significantly lower in autumn 2020 than in winter 2020 and spring 2021.
Conclusion: SARS-CoV-2 infection can occur in children with no clinical symptoms. In addition, the infection rate is in direct correlation with an increase in age of the children.
