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Showing 2 results for Staphylococcus Aureus

Gh.r Jahed Khaniki , A Kamkar , M.a Tehrani ,
Volume 3, Issue 3 (6-2005)
Abstract

A study was carried out to estimate the prevalence of Coagulase – Positive Staphylococcus aureus in milk collected in the milk collecting center in Garmsar city. Ninety-six raw milk samples (84 composite samples and 12 bulk samples) were collected from Garmsar milk collecting center. Raw milk samples were transferred to laboratory in safe conditions. After serial dilution, the appropriate concentrations (10 -2 to 10 -6) were cultured on Baired- Parker agar medium. The plates were incubated at 37 oC for 2 days (48h). After 48 hours' incubation, the colonies with black centers and light areas were used for staphylococcal assay. The coagulase test was done with rabbit plasma and coagulate- positive colonies were then counted. The results showed that mean counts of coagulate-positive Staphylococcus aureus in composite and bulk milk were 5.4 ×10 5 cfu/ml and 3.2 × 105 cfu/ml, respectively. The counts ranged from 8.6 × 10 4 to 2.1 × 10 6 cfu/ml and 1.4 ×10 5to 5.5 × 10 5 cfu/ ml. The standard deviations were 3.5 × 10 5 cfu/ml and 1.4 × 10 5 cfu/ml respectively. It is clear that the milk collected at this facility was rather heavily contaminated with S. aureus. Therefore, it may constitute a health hazard because of the possible production of enterotoxin in the milk consumed by the people. In order to reduce staphylococcal contamination in milk, it is recommended to treat cases of clinical and subclinical staphylococcal mastitis in livestock. Also, it is necessary to use hygienic procedures during the production process as well as transportation and storage.
S Bagherzadeh Yazdchi, M.h Pourmand , M Hajiabdolbaghi, M Hoseini , N Mardani ,
Volume 6, Issue 2 (7-2008)
Abstract

Background and Aim: Staphylococcus aureus is one of the most important human pathogens. A major clinical concern is the high rate of antibiotic resistance among Staphylococcus aureus strains. Methicillin-resistant S. aureus (MRSA) strains are a major cause of community- and hospital-acquired infections. Therefore, genetic investigation of S. aureus strains isolated from patients with staphylococcal infection is crucial not only for tracking of infections but also for nosocomial infection control.
Materials and Methods: In this study, 103 Staphylococcus aureus isolates were collected from Tehran University of Medical Sciences hospitals. Antibiotic susceptibility patterns of the isolates were determined using a panel of 13 antibiotics by the disk-diffusion method. The isolates were investigated for hvr by the PCR method.
Results: According to the antibiotic susceptibility testing, 21 antibiotype profiles were detected. Sixty-four out of the 103 isolates were resistant to methicillin. PCR results showed 10 different patterns of hvr.
Conclusion:
The results of this study showed high rates of antibiotic resistance and variations of hvr among the Staphylococcus aureus isolates. The high degree of hvr variation can be a good tool to use for molecular typing of this bacterial strain. Further investigation of antibiotic susceptibility patterns and genetic features of this bacterium is highly recommended for controlling community- and hospital- acquired infections.

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